Molecular cloning expression purification and characterization of 946 mannanase of family 26 glycoside hydrolase and associated family 35 carbohydrate binding module from clostridium thermocellum and its application in manno oligosaccharides production

Abstract

Plant cell wall is predominantly composed of complex structural polysaccharides such as celluloses and hemicelluloses Polysaccharides of the primary cell wall components of plant are cellulose hemicelluloses such as xyloglucans mannans galactomannans glucomannans laminarin glucoronoarabinoxylans arabinoxylan etc Mannans are the polysaccharides with a backbone chain of 206 178 1 4 linked mannose units They constitute a major portion of hemicelluloses in hardwoods The major distribution