Influence of low density lipoprotein receptor gene polymorphisms proprotein convertase subtilisin Kexin 9 and Apo B in coronary artery disease

Abstract

ABSTRACT newlineIntroduction and aim: Coronary Artery Disease (CAD) is a multifactorial newlinecondition caused by the interplay of hereditary and environmental factors. The newlinemajor objective of this research is to compare the conventional risk factors and newlinebiochemical parameters between coronary artery disease patients and normal newlinecontrols. To compare the plasma levels of PCSK 9 and Apo b in CAD and newlinenormal controls. Also to assess the PCSK 9 , Apo B and HbA1C levels in CAD newlinewith and without diabetes mellitus.To analyze single nucleotide polymorphisms newline[SNP]-rs688 and rs5925 of LDLR gene and to study its relationship with newlinedemographic andbiochemical risk factors in CAD.The research included 62 newlinepatients with coronary artery disease and 62 healthy controls.to examine the newlinelipid profile ,and diabetic parameters fasting venous blood was taken .Genomic newlineDNA was isolated ,amplified and identified using an allele specific polymerized newlinechain reaction for the genetic study of LDLR SNPs. Results: lipid profile newlineparameters were shown to substantially linked with coronary artery disease. newlinePCSK 9 values were significantly correlated with this disease. There was, newlinehowever, no significant change in allele and genotype frequencies of the LDLR newlineSNPs rs688 and rs5925 between the case and control group investigated. newlineConclusion: The current investigation was able to detect a significant newlinecorrelation between lipid profile parameters, diabetic parameters and PCSK 9 newlinewith CAD. But unable to detect a significant connection between the LDLR newlinegene SNPs rs688 and rs5925 with CAD. newline