Effect of Iron Overload Induced Oxidative Stress on Vital Organs and Assessment of Efficacy of Bioflavonoids in Overcoming Oxidative Stress

Abstract

The secondary iron overload conditions are encountered due to non-genetic newlinepredisposition; it is associated with several physiological complications which leads to newlinedevelopment of oxidative stress and also affects the functioning of vital physiological newlinesystems. Semi synthetic microbial siderophores like deferoxamine mesylate (DFO) are newlineprophylatically used to treat iron overload related complications. Continuous infusion of newlineDFO reported with several acute and chronic adverse drug reactions (ADR). In the newlinepresent investigation our primary focus was to evaluate the efficiency of polyphenolic newlinebioflavonoids during iron overload condition. The objectives were achieved in three newlinephases. The phase-1 of the study included screening and standardization of polyphenolic newlinenutraceuticals by principles of design of experiments. The Plackett-Burman fold over newlinedesign and Taguchi orthogonal methods were used to evaluate and validate the screening newlineprofile by ferric reducing antioxidant power assay (FRAP). This study concluded that newlinecurcumin (C), gallic acid (G) and quercetin (Q) are having potent antioxidant potential newlineagainst free radicals generated by ferric-TPTZ (tripyridyl hydrazine) complex at newlineequimolar ratio and they have exhibited the synergistic activity. This combination was newlineabbreviated as CGQ. However, the TLC densitometric study also exhibited that screened newlinenutraceuticals CGQ are non-interacting in the combination. The phase-2 of the study used newlineto evaluate the direct iron chelation properties of screened nutraceuticals. The newlinenutraceutical ligands and their corresponding iron co-ordination complexes were prepared newlinealone and in combination. The analysis by energy dispersive spectroscopy (EDX) and newlinedifferential scanning colorimetry (DSC) revealed that the resultant iron-nutraceutical newlineligands has a potential iron chelation property as that DFO and the nutraceuticals in newlinecombination possess better iron chelation property than their individual nutraceutical newlinecounterparts. The confirmatory, phase-3 where in vivo inves