Effect of cryopreservation on semen biochemical parameters including lipid profile in beetal and Assam hill goat

Abstract

A total of 72 ejaculates comprising six ejaculates from each of 12 bucks (six Beetal newlineand six Assam Hill Goat) were used to study the effect of cryopreservation on the physical newlineand biochemical characteristics of semen including sperm ultrastructure. The physical newlinecharacteristics of fresh semen viz., ejaculate volume, mass activity, initial sperm motility, newlinelive sperm, sperm concentration, cold shock resistance index, acrosomal integrity, HOSTreacted newlinesperm and sperm abnormalities were studied by conventional methods. The newlinebiochemical characteristics viz., sodium, potassium, calcium, total cholesterol, total lipid, newlineAST, ALT and lipid profile were studied in seminal plasma, and total cholesterol, total newlinelipid and lipid profile were also studied in spermatozoa of fresh and frozen semen. Semen newlinewas extended in Optixcell extender and frozen in 0.25 ml straws using liquid nitrogen newlinevapour and stored in liquid nitrogen. Each sample was evaluated on the following day for newlinesperm motility, live sperm, acrosomal integrity and HOST-reacted sperm. The newlinebiochemical characteristics studied after freezing in extracellular medium were the same newlineas in case of seminal plasma. Sperm ultrastructure was studied both in fresh and frozenthawed newlinespermatozoa. newlinePhysical and biochemical characteristics of semen of Beetal and Assam Hill Goats newlinewere within normal range and differed significantly between breed for ejaculate volume newline(Plt0.001), sperm concentration (Plt0.001), cold shock resistance (Plt0.05), Host-reacted newlinesperm (Plt0.01), sodium (Plt0.001), potassium (Plt0.001) and total lipid (Plt0.001). Postthaw newlinesperm parameters viz. sperm motility, live sperm, acrosomal integrity and HOST newlinereacted sperm, and content of biochemical constituents viz. sodium, potassium, calcium, newlinetotal cholesterol and total lipid decreased after freezing in semen of both the breeds, while newlineAST and ALT increased. Thirteen and fourteen fatty acids were identified in the seminal newlineplasma of Beetal and Assam Hill Goat bucks, respectively. Pentanedioic acid was newlineidentified only in seminal plas