Experimental and theoretical Validation of antioxidant activity of flavonoids from secondary Metabolites

Abstract

Secondary plant metabolites have been used in traditional medicine newlineand folk medicine to treat a variety of ailments. They offered lead compounds newlinefor the manufacture of drugs to treat a variety of disorders ranging from newlinemigraine to cancer in modern medicine. Human society is one among the newlineliving groups in this planet which gains potential benefits from the secondary newlinemetabolites derived from plant sources majorly. Flavonoids are one among newlinethe secondary metabolites having diversified role from protecting plants from newlineharmful Ultra Violet radiations from the Sun to protecting human beings from newlinehealth disorders like coronary heart diseases, cancer, diabetes etc., newlinePresent work focuses on identification and validation of antioxidant newlinebehaviour of flavonoids from medicinal plants of Western Ghats. Three plants newlinenamely Macroptilium lathyroides (M. lathyroides), Terminalia arjuna newline(T. arjuna) and Centella asiatica (C. asiatica) which are having wide newlinespectrum of usage by tribal people of Western ghats are chosen for the study. newlineCollection of medicinal plants are newlineSeeds are chosen as source of secondary metabolites from M. newlinelathyroides, bark is chosen from T. arjuna and whole plant of C. asiatica newlineitself was chosen to be the sources of secondary metabolites. All these sources newlineare shade dried and powdered. Using the dried content different solvent newlinefraction are taken with increasing polarity (Water-Ethyl acetate-Methanol and newlineEthanol). These fractions are further taken for qualitative and quantitative newlineanalysis for flavonoid identification. According to TPC and TFC analyses, the newlineethanolic and ethyl acetate extracts of M. lathyroides had larger quantities of newlinephenolic components, particularly flavonoids. newline