Effects of Moringa oleifera Extract Phytosteroids on Mystus gulio Gonad Development

Abstract

The MSE extract contains around 42 percent of phytosteroids. The experiment -I the newlinehistological observation of gonad shows high maturation having the highest oocyte diameter newline(856.69±29 µm) and GSI value of female M. gulio (30.49±3.5 %) in T3 treatment (1g/kg MSE). newlineThe Vtg concentration in T3 was also high in both serum and ovary confirmed by ELISA and newlinewestern blot analysis. In both exp-I and II. The level of total serum protein, hemoglobin, and newlinetotal RBC count was significantly (plt0.05) high in T3 as compared to control. Whereas high newlineconcentration (1.5g kg-1 MSE) shows lipid deposition (vacuolation) and increases the newlineintercellular space of cells in both intestine and liver tissue. This higher dose decreases the newlineoocyte maturation by showing less amount of Vtg in the ovary of females and a decline in newlinespermatogenesis of male M. gulio. The SeNPs were crystalline spherical in the structure having newline50 to 200 nm in size. Both the moringa and SeNPs contain a good number of antioxidants newlinewhich together reduce the ROS in M. gulio conformed to the stress enzyme parameter viz SOD newline(0.238±0.39 U/ml), catalase (34.38 ± 4.6 nmol/ml), and cortisol (2875.3 ± 34 pg/ml). Further newlinesignificantly (plt0.05) increases the hemoglobin level (10.80 ± 0.40 g/dl), Growth % newline(185.4±0.48), total serum protein (3.73±0.02 g/dl), survivability rate (78.6 ±0.76 %) in M. newline