Shodhganga : a reservoir of Indian theses @ INFLIBNET
The Shodhganga@INFLIBNET Centre provides a platform for research students to deposit their Ph.D. theses and make it available to the entire scholarly community in open access.
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10603/479511
| Title: | Cloning expression and purification of epidermal growth factor receptor for aptamer selection for certain bioassays |
| Researcher: | Sambhavi |
| Guide(s): | Bora, Utpal |
| Keywords: | Life Sciences Microbiology |
| University: | Indian Institute of Technology Guwahati |
| Completed Date: | 2016 |
| Abstract: | The present study demonstrates the selection and characterization of DNA aptamers specific to the extra cellular domain ECD of Epidermal Growth Factor Receptor EGFR protein In this pursuit the ECD of EGFR was cloned expressed and purified and a panel of DNA aptamers binding specifically to EGFR protein was selected by Systematic Evolution of Ligands by EXponential enrichment SELEX Then the candidate aptamers were sequenced and their potential secondary structure was predicted by mfold |
| Pagination: | Not Available |
| URI: | http://hdl.handle.net/10603/479511 |
| Appears in Departments: | DEPARTMENT OF BIOSCIENCES AND BIOENGINEERING |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 01_fulltext.pdf | Attached File | 18.44 MB | Adobe PDF | View/Open |
| 04_abstract.pdf | 82.13 kB | Adobe PDF | View/Open | |
| 80_recommendation.pdf | 860.91 kB | Adobe PDF | View/Open |
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