Formulation of neem oil derived nano emulsion by low energy method and its characterisation

Abstract

S.Pyogenus is better than raw neem oil, whereas inferior than Ampicillin drug. newlineBesides this, a study was also carried out to prepare neem oil-in-water nano-emulsions stabilized by Brij 30 surfactant using the phase inversion temperature (PIT) method at three different temperatures, i.e., 60°C, 75°C and 80°C. Compositions of homogenous phase were identified in the pseudo-ternary phase diagram. Among the total seventeen formulations, three formulations (NB1, NB2 and NB3) were short-listed and characterized for emulsion size and viscosity. The selected formulations showed emulsion size of 348-981 nm in diameter. The volume percentage ratio of Brij30 to neem oil showed significant effect on the droplet size of nano-emulsions. Formulations having lower concentration of Brij 30 displayed a smaller emulsion droplet size (348 nm). The NB3 formulation (4% neem oil, 11% Brij 30 and 85% deionized water) exhibited the highest stability after 60 days of storage. Antimicrobial study showed that in contrast to raw neem and Ampicillin (synthetic drug), NB1 exhibited best result in terms of minimum inhibition concentration (MIC) reduction by 100% against E-coli, P. Aeruginosa and S.Aureus and S.Pyogenus. newline