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http://hdl.handle.net/10603/472895
Title: | Cytoskeletal filament and intracel lular cargo dynamics facilitated by motor proteins role of activity and a catch bonded dynein |
Researcher: | Gupta, Nisha |
Guide(s): | Chaudhuri, Abhishek |
Keywords: | Physical Sciences Physics Physics Particles and Fields |
University: | Indian Institute of Science Education and Research (IISER) Mohali |
Completed Date: | 2019 |
Abstract: | The cytoskeleton of the cell is a highly complex, dynamic and adaptive system newlineof semiand#64258;exible and#64257;laments with their associated motor proteins and cross-linkers. It newlinegenerates internal stresses and responds to external ones, intermediates cell signal- newlineing, ensures the structural integrity and morphology of the cell and helps in the newlinespatial organization of cellular contents. This dynamic regulation is eand#64256;ected by the newlinecoordination of the diand#64256;erent cytoskeletal components : polymeric and#64257;laments such as newlinemicrotubules, actin and intermediate and#64257;laments; cross-linker proteins such as fascin newlineand and#945;-actinin; and motor proteins such as dynein, kinesin and myosin. newlineMuch of the physical understanding of the components of cytoskeletal network newlinehave been obtained through in-vitro experiments on cytoskeletal extracts. In a bead newlineassay a cytoskeletal and#64257;lament is irreversibly attached to a substrate, while the motor newlineproteins moving on the and#64257;lament are attached to a bead which is optically trapped. newlineOn the other hand, in a gliding assay setup, the geometry is inverted. Here, the newlinecargo domains of motor proteins are irreversibly adsorbed on a glass substrate, while newlinetheir and#64257;lament binding domains attach to the complimentary cytoskeletal and#64257;lament. In newlinethe presence of ATP, the motors move in a directed fashion along the length of the newlineand#64257;lament before detachment. This results in a gliding movement of the cytoskeletal newlineand#64257;lament in the opposite direction. Apart from a wealth of information on single motor newlineprotein movement and force generation, motility assays have revealed the emergence newlineof collective motion in high densities of such and#64257;laments leading to the formation of newlineclusters, swirls and interconnected bands. Within these in-vitro settings, it is possible newlineto ask theoretical questions regarding both the role of individual motor proteins and newlinetheir cooperative dynamics, in controlling the dynamics of the cargo/and#64257;lament that newlinethey bind/unbind to/from and therefore make falsiand#64257;able predictions amenable to newlineexperimental veriand#64257;cations. |
Pagination: | 90p. |
URI: | http://hdl.handle.net/10603/472895 |
Appears in Departments: | Department of Physical Sciences |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
01_title.pdf | Attached File | 340.07 kB | Adobe PDF | View/Open |
02_preliminary pages.pdf | 417.7 kB | Adobe PDF | View/Open | |
03_contents.pdf | 60.17 kB | Adobe PDF | View/Open | |
04_abstract.pdf | 60.77 kB | Adobe PDF | View/Open | |
05_chapter 1.pdf | 562.96 kB | Adobe PDF | View/Open | |
06_chapter 2.pdf | 729.94 kB | Adobe PDF | View/Open | |
07_chapter 3.pdf | 1.64 MB | Adobe PDF | View/Open | |
08_chapter 4.pdf | 722.92 kB | Adobe PDF | View/Open | |
09_chapter 5.pdf | 585.93 kB | Adobe PDF | View/Open | |
10_chapter 6.pdf | 93.48 kB | Adobe PDF | View/Open | |
11_annexures.pdf | 116.34 kB | Adobe PDF | View/Open | |
80_recommendation.pdf | 395.03 kB | Adobe PDF | View/Open |
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