Pathological and molecular studies on respiratory Diseases in chicken with special reference on Infectious bronchitis and infectious Laryngotracheitis

Abstract

The present study was conducted to understand the pathotype and genotype of viral strains of Infectious laryngotracheitis (ILT) and Infectious bronchitis (IB) that caused high morbidity and mortality in the poultry flocks and involved in the disease outbreaks, by pathological and molecular approaches such as immunohistochemistry, genome sequencing and ultrastructural studies. Keeping in view, the paucity of data on the detection and distribution of ILT and IB viral antigens in Tamilnadu, the farms in and around Namakkal, Tiruchirapalli, Pudukkottai and Thanjavur districts of Tamil Nadu with suspected respiratory diseases were selected for this study. The data pertaining to the breed and strain of chicken, flock strength, age, method of rearing, production performance and clinical signs manifested were recorded. newlinePrevalence of ILT and IB diagnosed by pathology, immunohistochemistry, ultrastructural pathology, virus isolation, PCR sequencing and molecular characterization inferred that different forms of genotypes and pathotypes of ILTV and IBV variants were under circulation among the chicken flocks in Tamil Nadu during the study period and these variants were targeting the reproductive system and lymphoid organs along with the respiratory system. newlineKey words: Chicken, respiratory diseases, ILT, IB, pathology, newlineimmunohistochemistry, PCR, phylogenetic analysis and ultrastructural pathology. newline newline