Studies on the Effect of and#914;eta Caryophyllene on Insulin Signaling Molecules in Gastrocnemius Muscle of High Fat Diet and Fructose Induced Type 2 Diabetic Experimental Rats

Abstract

Abstract: newline newlineHigh fat diet feeding results in hyperglycemia and insulin resistance which is a major pathological feature of type-2 diabetes mellitus. Usages of oral hypoglycemic drugs are limited due to its deleterious side effects and there is a need to find more efficacious agents for diabetes management. Hence, in the present study we attempts to elucidate the mechanism of action of and#946;-Caryophyllene on insulin signaling molecules in gastrocnemius muscle of high fat diet- inducedtype-2 diabetic rats. An oral effective dose of with and#946;-Caryophyllene (200 mg/kg b.wt) was given for 30 days to high fat diet (comprising 2% cholesterol, 1% cholic acid, 30% coconut oil, 67% conventional rat feed) and fructose fed type-2 diabetic rats to find out whether and#946;- Caryophyllene regulates IRS 1/Akt pathway of insulin signaling. The data obtained in the present study elucidated that, and#946;- Caryophyllene treatment significantly increased the RNA and protein expression of insulin receptor (IR) in diabetic rats whereas there is no significant difference inmRNA expression of insulin receptor-substrate-1 (IRS-1) was observed among groups. The Akt mRNA and GLUT-4 mRNA and protein level were also improved in gastrocnemius muscle oftype-2 diabetic rats. Therefore, we concluded that and#946; Caryophyllene could be used as potential phyto medicine fortype-2diabetes management. newline newline