Aanti fungal susceptibility of malassezia to azoles by broth microdilution method and its phylogenetic relationship based on multi locus sequence analysis

Abstract

Malassezia spp colonizes the human skin after birth and acts as a commensal normally tolerated by human immune system Malassezia though known for its cutaneous infections can potentially cause invasion The skin infections caused by Malassezia has poor patient compliance due to its chronicity and recurrent nature of the disease There is also a lack of standardized anti fungal susceptibility profile for Malassezia due to its complex growth requirement and also variation in anti fungal susceptibility among the species This emphasizes the need to identify the species causing infection Considering the importance of Malassezia in medical mycology this study was done to know the epidemiological pattern of Malassezia in our locality and to identify the species causing the infections by phenotypic and molecular methods and to conclude with the best drug drugs to treat the infections caused by Malassezia Skin samples were collected processed species identified by conventional method AFST done by broth micro dilution method DNA was extracted polymerase chain reaction PCR was done. The product was sequenced and species identified The epidemiological pattern showed adolescent female commonly affected in torso The commonest lesion was pityriasis versicolor Malassezia sympodialis was the commonest species isolated Among the anti fungals used fluconazole miconazole showed high MIC values and itraconazole clotrimazole luliconazole and sertaconazole showed low MIC values The systemic anti fungal of choice was itraconazole with the lowest MIC Minimum Inhibitory Concentration of 0 point 125 1and#956;g ml The best topical drug with lowest MIC value was clotrimazole0.03 to 0 point 5 and#956;g ml newline