Determination of prevalent genotype of hepatitis B virus and their corresponding resistance pattern in Jaipur region

Abstract

HBV infection is a major global health issue that places a continuously rising newlineburden on emerging countries like India. Chronic hepatitis B virus infection is newlinelinked to an increased risk of liver disease, such as cirrhosis and hepatocellular newlinecancer. The clinical appearance and natural history of HBV infection is mediated newlinethrough complex interactions between the virus and the host immune response. newlineViruses with mutations in the core or precore gene have an effect on replication and newlinemay enhance liver damage. newlineThe present study was planned to detect the prevalent HBV Genotypes and newlinedrug resistant mutations and their correlation by polymerase gene sequencing in newlinepatients with high HBV viral load at Jaipur, Rajasthan. newlineThe following mutations in RT region of polymerase gene L80V, V173L, newlineL180S/M, S202I and M204I were reported to be associated with HBV genotype D newlinein the present study. Antiviral therapy with nucleoside analogues (NAs) is a good newlinecontrol measure, but it requires long-term treatment. The use of nucleos(t)ides is newlinefrequent among Indian physicians. The restricted number of NAs and the emergence newlineof drug-resistant mutations in the HBV reverse transcriptase (rt) domain during newlinelong-term treatment leading to treatment failure is the major concern. newlineIn this research we planned to study the drug resistance to various antiviral newlinedrugs by sequencing to know common mutations prevalent in Jaipur and correlation newlineof drug resistance to genotypes for this blood samples were collected in EDTA vial newlinefrom patients attending SMS hospital and processed. Plasma was separated, labelled newlineand stored at -200 C. 1831 HBsAg positive patients were enrolled in the study. DNA newlinewas extracted by Roche High Pure System COBAS TaqMan kit and quantified by newlineCOBAS TaqMan HBV Test Kit on COBAS TaqMan 48 analyzer (Roche). Real newlineTime PCR (Roche) done to estimate viral load as per manufacturer s instructions newlinebecause high viral load is inclusion criteria in present study. newline newline