Role of beneficial bacteria on yield of Calocybe indica and Pleurotus species and management of contaminants in mushroom beds

Abstract

Milky (Calocybe indica) and oyster mushroom (Pleurotus spp.) is the newlinemostimportant cultivated mushroom in India and a very less works has been conducted newlineto identified the role of bacteria on growth and yield of mushrooms. Therefore, the aim newlineof present study was to investigate the effective bacterial spp. isolated from cultivated newlinemushroom substrates and their role in mycelial growth, influence of primordia newlineformation, pinhead and yield of mushrooms. Six bacterial cultures were isolated from newlinethe growth substrates of Pleurotus spp. and casing soil of Calocybe indica. Out of six newlinebacterial isolates, 3 isolates were observed positive effect on growth and development newlineof mushroom species. The other isolates were inhibiting the mycelial growth of newlinePleurotus florida, Pleurotus djamor and Calocybe indica. In in vitro experiment was newlineobserved that addition of effective single and combine bacterial isolates with pure newlineculture of C. indica, P. florida and P. djamor enhanced the pinhead formation in newlinePetriplate conditions as compared to control. In vitro cultivation of Pleuortus spp., newlinesingle and combined application of MB1, MB2 and MB5 could enhanached the newlineformation of pinhead, primordia and fruting body as well as increasing the yield of newlineP. florida and P. djamor. In in vivo experiments, combined application of MB1, MB2 newlineand MB5 (T7) isolates were found to be the best treatment to enhances the spawn run, newlineearly initiation of pinhead, formation of fruiting body and yield of C. indica and newlineP. florida. In case of P. djamor experiments, the maximum growth and yield parameters newlinewere observed in spraying of MB1 at 30 ml/bed (T1) followed by spraying of MB1, newlineMB2 and MB5 at 30 ml/bed (T7) to promote the growth and yield parameters of newlineP. djamor over control. The bacterial isolates viz., MB1, MB2 and MB5 were recorded newlinemore or less inhibition against the mycelial growth of Trichoderma spp. in in vitro newlineconditions. The mMilky (Calocybe indica) and oyster mushroom (Pleurotus spp.) is the newlinemostimportant cultivated mushroom in India and a very less ...