Genetic Manipulation of Sgt1 for Regulated Production of a Solanine in Potato Solanum tuberosum

Abstract

Solarium tuberosum is commonly known as potato, a highly consumed non-grain crop plant that is very popular for various products. Potato has many nutritional values but has two important secondary metabolites known as o-Solanine and e-Chaconine. These metabolites are worked as a plant defence mechanism and produce a high amount of metabolites when stress is detected. According to the researchers, when these glycoalkaloids concentration goes up to a certain level, it may cause certain neurological and gastrointestinal impairments. e- Solanine and o-Chaconine work in a co-dependent manner. Acetylcholinesterase is an essential enzyme and, at the postsynaptic cleft, splits the acetylcholine-signalling molecule into acetyl + Choline; e-Solanine and o-Chaconine inhibit the acetylcholinesterase. To understand how these two glycoalkaloids bind with acetylcholinesterase. We performed molecular docking analysis to analyse at which site and know which confirmation ligands are bound with the AChE. The analysed result showed the highest docking score off-Solanine (-16.645 Kcal/Mol) using acetylcholine and donepezil as reference molecules. In addition, perform molecular dynamics simulation for confirmation change analysis during simulation, using Schrodinger Maestro software. newline newlinePotato is a highly edible non-grain crop. It would be good to lower the effect of glycoalkaloids. Based on preliminary research analysis, only one glycoalkaloid e- Solanine was targeted for downregulation without harming the plant s biological defence system. This study targets the enzyme UDP-galactosyltransferase that adds sugar moieties to the precursor of e-Solanine called solanidine. Sgtl gene coded UDP-galactosyltransferase; for downregulation of the o- Solanine, sgtl was targeted using CRISPRi/Cas9. newline