Development of Organic Inorganic Hybrid Catalysts as a Dual Sensor for the Detection of Heavy Metal Ions and Biomolecules

Abstract

In order to avoid environmental contamination, there need of avoiding heavy metal ions (HMIs) and biomolecules (BMs). For that, DNAzymes and Nanozymes are promising candidate detect HMIs and BMs, which enhance the detection feasibility than commercial nanomaterials. Hence we aim to develop a promising functional materials, for that we have synthesized magnetic porous carbon (MPC), magnetize nanoscale metal-organic framework (MNMOF), and polyaniline nanoclips (PANCs). The synthesized organic inorganic hybrid materials (OIHMs) were confirmed with XRD, FT-IR, FE-SEM, TEM, UV-Vis, VSM, BET and XPS. Especially, sensing studies has estimated by photoluminescence (PL) and UV-Visible spectroscopy (UV-Vis). The FAM-labeled single strand DNA (FAM-ssDNA) adsorbed on the surface of OIHMs through and#960;-and#960; stacking, electrostatic interaction and hydrogen bonding. As result, the fluorescent emission was decreased because immobilization of ssDNA. The fluorescent efficiency were achieved 96%, 62% and 91% for MPC, MNMOF, and PANCs. Interesting note that, MPC have a strong adsorption for As (V) over than ssDNA. However, fluorescent emission intensity has retained. As result this system working based fluorescence turn off and on strategy. According to that, we reached low detection limit was 630 pM. This hypothesis emphasis MPC have selectivity and sensitivity towards As (V) over than other competitive metal ions. Moreover, PANCs have a strong adsorption property towards FAM-ssDNA through non-covalent interactions. So that, fluorescence intensity decreased due to PET newline