Synthesis spectral antimicrobial and dna binding studies of thiazolidin 4 one containing heterocyclic compounds

Abstract

In the present investigation, synthesis and characterization of thiazolidin-4-one newlineincorporated 2,6-diarylpiperidin-4-one, 2,6-diarylthan-4-one and 3-azabicyclononanes, and newlinetheir application on pathogenic bacteria were investigated. All the compounds were newlinesynthesized by conventional and microwave irradiation in presence of Amberlite IR-120H newlineresin. The detail characterization of the compounds were carried out using Elemental newlineanalaysis, FT-IR, 1H, 13C, COSY, NOESY, HSQC and HMBC NMR spectroscopy methods. newlineSingle crystal X-ray crystallographic study was also carried out. newlineThe observed chemical shifts and coupling constants clearly indicates that the newlinesynthesized 2.6-diarylpiperidin-4-one, 2,6-diarylthan-4-one compounds were presents normal newlinechair conformation with equatorial orientation of the aryl groups at C-2 and C-6. Moreover, newlinethe chemical shifts strongly suggested that the synthesized compounds were result in single newlineisomer i.e., E isomer. In vitro antitubercular activity of synthesized compounds was carried newlineout against Mycobacterium tuberculosis H37Rv by Microplate Alamar Blue Assay method newline(MABA). Isoniazid was used as standard drug. This study reveals that halogenated newlinecompounds showed good tubercular activity. newlineIn vitro antibacterial and antifungal activities were carried out against tested bacterial newlineand fungal strains. Streptomycin and Amphotericin B were used as standard drugs. This study newlinereveals that all compounds showed moderate to good activity against all the tested organisms. newlineThe interaction between the compounds and CT-DNA was studied by UV-visible, newlineFluorescence spectra, CD, cyclic voltammetric, melting denature and viscosity measurements newlinemethods. This study revealed that the mode of binding was confirmed as non intercalative. newline