Characterization of Metabolites from Marine Actinomycetes and their Bio Potential Applicants

Abstract

12 marine derived actinomycetes were isolated from sediment of cochin port collected around 7 different location followed by Pre-treatment of sample. The data of physico-chemical characteristics like Bulk density, TOC, nitrogen, micronutrients, salinity, conductivity and pH values were varied among stations. The phenol pre-treated soil shows reduced viability Streptomycetes while the dry heat treatment drastically reduced the number of bacteria without reducing the Streptomyces spp population. Genera of isolates identified as Streptomyces spp (STR) Micromonospora spp (MM) Nocardia sp (NOC) Micropolyspora spp (MP). The frequency of non streptomyces was 25% in untreated sample further improved to 100% by phenol treatment. Heat treated sample showed recovery of Streptomyces from 75 to 83%. Out of 12, 25and#8805;8and#8805; 41 and 33 percentage of them were detected as phosphate solublizer, NH3 producer, IAA producer and cyanide producer. Plant growth trait Streptomyces sp STR3 on V.radiata have given 1856 vigour index under induced drought condition. The actinomycetes were subjected to primary screening and 33 % were positive on antibiotic producer under ISP4 broth. Among the 12, isolates STR3 and 7, MM4 and MP2 found to be Antibacterial compound producers. Among the four strains, STR7 only showed antimycobacterial activity (16 mm/ 12.5 and#956;g MIC) and the GCMS reveals novel compounds. Based on ADME Caffeine, Nortrachelogenin and 1,2-Benzenedicarboxylic acid were docked with Acyl-CoA Carboxylase and found good binding affinity to inhibit the enzyme. Asparaginase production using a qualitative rapid plate assay reveals that MM4 which were producing large L-asparagine hydrolysis zones. A promising isolate, MM4 was identified by 16S rRNA gene sequencing as M.citrea Maximum L-asparaginase production was recorded in starch amended wit casein. An SDS-PAGE analysis of the purified ASNase showed a single distinct protein band with a molecular weight of 70 kD. The free radical scavenging activities were accessed by Hydroxyl, DPPH, and FRAP radic