Multivalent glycoconjugates to modulate carbohydrate protein interactions cpis

Abstract

Carbohydrate protein interactions CPIs are one of the most important and major events on cell surfaces Basic components involved in the interaction are the cell surface glycans which demonstrate a sensitive and selective cis trans binding with protein counterparts However due to weak CPIs nature facilitates multivalency to target specific proteins Recently extensive efforts have been directed toward mimicking these bio events by replicating the multivalent scaffolds Significant progress has been achieved in this regard using multivalent glycoprobes However other important issues regarding the preparation of multivalent carbohydrates are related to the size shape orientation and local concentration of the sugars with respect to external stimuli In the following chapters we have investigated the role of different shapes in carbohydrate mediated interactions Chapter 1 summarizes different multivalent scaffolds and their major applications in targeting carbohydrate protein interactions We highlighted the current efforts made in the synthesis of glycodendrimers glycopeptides metallo glycodendrimers glyconanoparticles and supramolecular complexes and the role of spatial arrangements chirality and symmetry of these multivalent probes in carbohydrate protein interactions Finally we discuss shape dependent biological interactions that have been reported in literature Chapter 2 describes the synthesis of glyco gold nanoparticles of three different shapes and their applications in bacterial aggregations and infection More specifically we have compared the behavior of nanospheres nanorods and nanostars with mannose and galactose conjugations The mechanism of aggregation revealed that the large number of surface interaction of rod shaped mannose AuNPs with E coli ORN 178 compared with spherical and star shaped AuNPs Moreover such sensitive binding can be used for effective inhibition of bacterial infection of cells Chapter 3 deals with the shape dependent uptake of glyco gold nanoparticles G AuNPs by dif newline newline