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http://hdl.handle.net/10603/458905
Title: | The role of clasps in clathrin assembly on membrane |
Researcher: | HOLKAR, SACHIN S. |
Guide(s): | PUCADYIL, THOMAS J. |
Keywords: | Biology Biology and Biochemistry Life Sciences |
University: | Indian Institute of Science Education and Research (IISER) Pune |
Completed Date: | 2016 |
Abstract: | Clathrin mediated endocytosis CME internalizes most of the membrane proteins cargo in eukaryotic cells Initiation maturation and fission are the sub stages during CME Early events initiation and maturation during CME involve clathrin associated sorting proteins CLASPs recruitment membrane bending and clathrin assembly on plasma membrane There are more than 100 molecules involved in CME including cargo adaptor proteins CLASPs accessory proteins and clathrin These CLASPs have weak affinity for specific cargo PIP2 accessory proteins AP2 and clathrin These complex interactions are responsible for functional redundancy of these CLASP molecules and show little effect on CME in cells Also limitation of specio temporal resolution makes it difficult to understand precise role of individual CLASPs during clathrin assembly reaction To address this we have used reconstitution approach by using defined set of protein molecules Current assays and reconstitution schemes show minimal machinery required to form clathrin coated pit on model membrane system but lack dynamics of the process My work is divided into two parts in the first part we have developed new assay system to study role of membrane curvature sensitivity of one of the indispensable adaptor molecules 8220 epsin 8221 in clathrin assembly Second part has comparative study of different monomeric adaptor proteins in clathrin polymerization Epsin is one of the essential CLASP molecules for clathrin mediated endocytosis We have developed a new assay system with budded supported membrane tubes SMrT Epsin shows membrane curvature sensitivity and sorts on highly curved membrane tubes in presence of phosphatidylinositol 4 5 bisphosphate PIP2 Fluorescently labeled clathrin clusters epsin molecules and assembles as foci on membrane tubes We have shown the importance of membrane curvature sensitivity of epsin during clathrin assembly reaction This assay system allows us to study real time clathrin polymerization dynamics on highly curve membrane tubes Time constant |
Pagination: | NA |
URI: | http://hdl.handle.net/10603/458905 |
Appears in Departments: | Department of Biology |
Files in This Item:
File | Description | Size | Format | |
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01_fulltext.pdf | Attached File | 6.6 MB | Adobe PDF | View/Open |
04_abstract.pdf | 444.08 kB | Adobe PDF | View/Open | |
80_recommendation.pdf | 546.04 kB | Adobe PDF | View/Open |
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