Some studies on Lobotes Surinamensis Bloch 1790 from Visakhapatnam Fishing Harbour East Coast Of India With Special Reference to DNA Barcode Proximate Composition Antioxidant and Antimicrobial Activity

Abstract

PREFACE newline newline Some studies on Lobotes surinamensis (Bloch, 1790) from Visakhapatnam Fishing newlineHarbour, East Coast of India with special reference to DNA Barcode, Proximate newlineComposition, Antioxidant and Antimicrobial Activity . This work establishes the newlinemolecular taxonomy of Lobotes surinamensis by using mitochondrial COI gene and newlinealso evaluates the nutritional quality and bioactive potentials by quantitative newlinedetermination of proximate composition, antioxidant, and antimicrobial activities. newlineLobotes surinamensis is a pelagic fish species found throughout tropical and subtropical newlineregions of the world s oceans. The taxonomic identification of species is crucial for the newlineassessment of proximate composition and bioactive potentials. This can be achieved by newlinethe amplification of mitochondrial C oxidase subunit I. The proximate composition of newlinea Lobotes surinamensis helps to assess its nutritional and edible value. The medicinal newlinevalue of the fish depends on the presence of bioactive compounds that exhibits newlineantioxidant properties to protect the body against harmful reactive oxygen and nitrogen newlinespecies which damage biomolecules by oxidation. The present thesis includes three newlinechapters. newlineChapter I deals with the identification of Lobotes surinamensis by using mitochondrial newlineCOI gene mediated molecular phylogeny. newlineChapter II establishes the proximate composition by quantitative determination of newlinebiomolecular components such as moisture, total ash, acid insoluble ash, total newlinecarbohydrate, total protein, total lipid, and total free amino acids. newlineChapter III establishes the medicinal importance of Lobotes surinamensis by newlinequantitative determination of antioxidant activates such as DPPH, ABTS, FRAP, H2O2, newlineand Superoxide scavenging activities. Furthermore, the antimicrobial activity was newlineevaluated by the well diffusion method against selected gram-positive and negative newlinebacteria, along with fungi. It is shown that the Lobotes surinamensis had good newlinenutritional and medicinal properties. newlinei newlineAndhra University, Visakhapatnam newlineGENRAL INTRODUCTION