Therapeutic Status of Aswagandharishtam in Cellular Model of Diabetic Neuropathy

Abstract

Diabetic neuropathies have multifactorial pathogenic mechanisms and hyperglycemia plays an important role in the pathogenesis of diabetic neuropathy. Many herbal drugs, siddha drugs, ayurvedic drugs are the most commonly used for managing diabetic complications like neuropathy and scientific validation, efficacy and safety are to be further assessed by well designed research. To know the neuroprotective role of Aswagandharishtam as preventive, ayurvedic and siddha medicines to treat symptoms of diabetic neuropathy in schwann cell model. In the present study, the neuroprotective activity of aswagandharistham was evaluated in Cellular model. The work involves phytochemical analysis, invitro anti-oxidant studies and neuroprotective assays including gene expression analysis. Aswagandharishtam, was found to have significant cytotoxicity against hyperglycemic schwann cells , thereby justifies its neuroprotective potential on par with the standard drug used for diabetic neuropathy, pregabalin. The hormone, insulin have crucial roles in myelination and peripheral nerve support via Schwann cell signaling. The relative expression of insulin levels was increased with increasing concentration of aswagandharistham. The increased production of insulin helps to modify myelin protein expression in diabetic neuropathy and vii hence, can treat the symptoms of the disease. The expression of NGF levels was increased with increasing concentration of test compounds, Aswagandharishtam and standard drug pregabalin. NGF treatment improves diabetic sensory neuropathy. Aswagantharistam has good antioxidant potentials proved by various assays. The current study results suggested that aswagandharishtam exhibits a neuroprotective role against hyperglycemia-induced schwaan cells. Aswagandharishtam has the potential to reinstate the neuroprotective effect against hyperglycemicinduced Schwann cells by accelerating the mRNA expression of BDNF, NGF, INsr genes. The drug alleviates BDNF and may regulate inflammation regulation in diabetic neuropa