Drug Release Analysis and Anticancer Potential of Tabebuia pallida Silver Nanoparticles Loaded Liposomes against Molt 3 Cells In Vitro

Abstract

The main aim of the present study is to synthesize and characterize metallic nanoparticles and synthesised nanoparticles of T.pallida loaded liposomes and to evaluate its antioxidant and anticancer potential. The drug release profile of the silver nanoparticles loaded liposomes were newlinealso analysed and the results are validated using various mathematical models. In phase I, the AgNPs are synthesized by sunlight mediated green synthesis and the AgNPs loaded are prepared newlineby a conventional thin film hydration method. The synthesized silver nanoparticles and silver nanoparticles loaded liposomes were characterized using various spectral techniques. The UV visible spectrum peak at 450 nm indicates the formation of AgNPs. EDAX spectrum indicates the newlinepresence of 65.94% of silver and the XRD pattern reveals that the synthesized silver nanoparticles newlineare Face Centered Cubic crystalline in nature. High encapsulation of silver nanoparticles into the newlineliposomes was observed and the high encapsulation effficiency indicates the strong interaction newlineexist between the silver nanoparticles and the lipids. FTIR spectrum reveals the various functional newlinegroups that are involved in the synthesis of AgNPs and formation of Liposomes. The average size newlineof the nanoparticles and AgNp loaded liposomes was 55.4 nm and 172.1 nm with a dispersity newlineindex of the synthesized polymer 0.33 and 0.381as identified by DLS analysis. The Zeta potential newlineof AgNPs and AgNPs loaded Liposomes were found to be -15.6 mV and -21.5 mV respectively. newlineIn vitro Drug Release Profile of AgNPs Loaded Liposomes were carried out using 3 different newlinepH:pH 5.5 (Mature Endosomes of cancer cells), pH 6.8 (Cancer Cells) and pH 7.4 (Blood). A high newlineAgNP release was found at a pH of 5.5 (mature endosomes of cancer cells) when compared to the others clearly indicate the targeted delivery of the AgNps. This drug release profile was validated using various mathematical models including, Zero Order Model, First order model, Higuchi model, Korsmeyer and peppas model and Hixson crowel mode