Bioactivity Studies on Cleistanthus collinus Roxb Benth Ex Hook F Leaf Extracts

Abstract

According to the report of Global Burden of Disease (GDB) 2013, newlineover 95 % of the human populations are suffering from health issues. In newlinesimple, 19 out of 20 people are diseased. The humanity is served with either newlinenatural products based or chemically synthesized drugs. Since, chemical newlineformulations are resulting in adverse side effects, the demand for natural newlineproduct based drugs are more. Hence, a native Indian tropical plant newlineCleistanthus collinus, (Oduvanthalai in Tamil) was selected for the present newlinestudy. The interesting part of the plant is, it is a poisonous plant commonly newlineused for suicidal purposes, the mechanism of toxicity is still unclear. At the newlinesame time, in recent decades, this plant is also widely reported for its newlinetherapeutic potentials (mostly with crude extractions). newlineThe present study was designed to isolate and purify the active newlineconstituents of the plant from its leaves and to analyze the bioactive newlinepotentials of the purified compound. Liquid liquid extraction and preparative newlinechromatography methods were employed. Cleistanthin A, an active newlineconstituent was isolated and purified. The purified compound, cleistanthin A newlinewas analyzed for its bioactive potentials (Antimicrobial, antioxidant, newlineanticancer, aquatic animals stress management and molecular docking newlineefficiency) were analyzed. Also, silver nanoparticles using the plant leaves newlinewere synthesized and analyzed for its bioactive properties like antimicrobial, newlineseed germinating, dye degradation, larvicidal properties etc. newlineCleistanthin A exhibited significant antimicrobial activity against newlineMethicillin Resistant Staphylococcus aureus (MRSA), Enterococcus sp. and newlineCandida sp. The dog tick s neurosecretory cells and muscle cells when newlinexii newlinetreated with cleistanthin A, the cells were bulged with 10 and#956;g concentration newlineand disintegrated at 20 and#956;g concentration along with increase in time. When newlineA549 lung cancer cell lines treated with cleistanthin A, the proliferation of newlinecell lines were at-most inhibited at 100 and#956;g/mL of cleistanthin A. 6.25 and#956;g/mL newlinewas calculated as the IC50