Prevalence of phosphorus and potassium solubilizing microbes in soil and their influence on growth yield quality and nutrient uptake in Broccoli and Cauliflower

Abstract

newline The research work was performed in the Department of Industrial Microbiology.The soil samples newlinewere collected from the rhizospheric region of different vegetable crops planted in central field newlineof SHUATS. Phosphorus and potassium solubilizing microbes were screened by employing newlinequalitative and quantitative efficiency assessment techniques. All of them were identified based newlineon biochemical characterization up to genus level. The isolates showing maximum zone and newlinesolubilizing efficiency were screened for further analysis. Further, two phosphorus solubilizing newlinemicrobial isolates as well as two potassium solubilizing microbial isolates were screened for newlinefurther study. The isolates were mass multiplied for field trial and their concentration was newlinedetermined in Pikovaskaya and Alexandrov broth medium. newlineNumerous treatment combinations were made to see the effect of microbial isolates either alone newlineor in combination on Broccoli and Cauliflower. Physico chemical properties of treated soil newlinesamples along with control was determined using standard protocol and was found maximum in newlinemicrobial treatment T10 having both phosphorus and potassium solubilizing fungus newline(Trichoderma viride and Aspergillus niger). Increase in microbial population of bacteria was newline(23×106 newline, 34×106 CFU/g) and fungi spores was (15×104 newline, 28×104 newline) in broccoli planted soil and newlinebacteria (27×106 newline, 39×106 CFU/g) and fungi spores (17×104 newline, 26×104 newline) in cauliflower soil increased newlinein treatment T10, in both the years in both crops in comparison to control soil samples. After 90 newlinedays of sowing, the plant growth, yield, quality and nutrient uptake parameters were analyzed newlineand was found to increase in microbial treatment T10 (PSF1and KSF). Chlorophyll content in newlinecase of Broccoli at 60 days of sowing was recorded (59.80 mg/ ml, 62.58 mg/ml) and in case of newlineCauliflower (49.40/100ml, 50.70mg/100ml). Enhancement in yield parameters such as diameter newlineof curd was (15.3cm, 15.66cm) and (16.8 cm, 17.4 cm) and weight of curd was (558.33g,515g) newlineand (611