Characterisation and assessment of genetic purity through grow out test and molecular markers of DCH 32 Cotton Hybrid

Abstract

newline Characterization of varieties/hybrids is essentials in view of release of varieties and newlinegenetic purity testing. Hence, an experiment was carried out at Main Agricultural Research newlineStation, University of Agricultural Sciences, Dharwad during 2006-08 for varietal newlinecharacterization and genetic purity testing through morphological, chemical, biochemical and newlinemolecular markers in DCH-32 hybrid cotton. newlineThe DCH-32 hybrid and its parental lines were grouped as cream (DS-28), yellow newline(DCH-32) and deep yellow colour petal, whereas anther colour were grouped into light yellow newline(DS-28) and yellow (DCH-32) and SB(YF)-425 colour but petal spot was absent in DS-28 newlinefemale parent. The DCH-32 hybrid showed more similarity (64%) with DS-28 female parent newlineand less (57%). The seeds subjected to peroxidase test for characterization. Based on the newlinecolour of the solution, genotypes were grouped as red, dark red and reddish orange colour. newlineAmong the parental lines and the hybrid, the DCH-32 (42.14%) was more responsive to GA3 newlineand 2, 4-D (94.07%) test. newlineThe DCH-32 hybrid and its parental lines were characterized with SDS-PAGE of total newlinesoluble seed protein and 27 RAPD primers. Seven RAPD primers produced male parent newlinespecific marker, which were used for genetic purity assessment in hybrid seed lots. The newlinegenetic purity of the 50 seed lots resulted through field GOT ranged from 75.7 (L27) to 98.7 newlineper cent (L45). The genetic purity of selected hybrid seed lots (L1, L2 and L3) assessed through newlineSDS-PAGE (91.5%, 80% and 94%), RAPD (90%, 78% and 93%) marker and field grow out newlinetest (92%, 81.6% and 96.5%) indicated there is similarity in the results indicating that newlinemolecular markers can be used as supportive test for GOT. Though, the genetic purity newlineassessment through molecular markers (Rs. 1442.50) is costly comparatively field GOT (Rs. newline708) and the time taken for the test is very less (44 hrs) for assessing genetic purity.