Evaluation of Antioxidant and Pharmacological Activity of Ficus Religiosa and Trigonella Foenum Graecum for The Management of Wound Healing Potential in Diabetic Rat

Abstract

Abstract: Ficus is an evergreen tree of family Moraceae. Ficus religiosa and Trigonella foenum graecum has been traditionally claimed that the leaves of Ficus religiosa and seeds of Trigonella foenum graecum are useful in the management of diabetic wound healing. The leaves of Ficus religiosa and seeds of Trigonella foenum graecum are reported to have anti-inflammatory, antioxidant, and antimicrobial properties. In the present study, diabetic wound healing activity of ethanolic extracts of Ficus religiosa and Trigonella foenum graecum was evaluated in rats using excision, and dead space model along with estimation of biochemical parameters. In the excision wound model, the period of epithelisation, scar area, percent wound contraction or closure was evaluated respectively. While in the dead space model, the efficacy of drugs against the proliferative and remodeling phases of wound healing was evaluated. In addition, GSH, Catalase and MDA estimation studies were employed to assess the efficacy of both the plants in vivo. The treatment of diabetic wound with oral and ointment containing ethanolic extracts of Ficus religiosa and Trigonella foenum graecum exhibited significant diabetic wound healing. The results were comparable to standard drug povidone iodine ointment, in terms of wound contraction, period of epithelisation and histopathological parameters. A significant antioxidant activity was observed as evidenced from decreased level of MDA and increased the level of GSH, catalase in extract treated animals as compared to control animals. newlineKeywords: Wound healing, Ficus religiosa (FR), Trigonella Foenum Graecum, (TF) antioxidant, excision wound, dead space wound. newline