Antidermatophytic potential of streptomyces albidoflavus stv1572a against trichophyton mentagrophytes detection of effective component and target studies

Abstract

Dermatophytosis is a superficial fungal infection of keratinized tissue, generally called newlineringworm or tinea infections. Trichophyton mentagrophytes are one of the predominant newlinepathogen causing tinea infection. Such fungal infections affect the quality of life of newlinepatients by different factors, including morbidity, long duration of illness and sociodemographic newlinefactors such as uncertain socioeconomic status, age, sex, overcrowding, newlineoccupation etc. Thus, causing a psychological burden due to its inefficacy in the newlineprolonged course of treatment. An increase in the prevalence of tinea infections newlinenecessitates a search for novel antidermatophytic drugs from unexplored natural newlineresources. newlineThe present study reports a newly identified antidermatophytic property of actinomycetes newlineisolated from wetlands soil. A total of 87 isolates of Streptomyces were screened for newlineantidermatophytic activity against T. mentagrophytes. The isolate with 100% newlineantidermatophytic activity was characterised and submitted to NCBI, GenBank as newlineStreptomyces albidoflavus STV1572a (MH050390). Antidermatophytic compounds newlineextracted from this strain were partially purified by chromatographic techniques. newlineAdditionally, it was characterised using UV-Vis spectroscopy, FT-IR spectroscopy, and newlineGC-MS analysis. The compounds identified using GC-MS analysis are phenol 2, 4-bis newline(1, 1-dimethylethyl), 9-octadecene (e), e-14-hexadecenal, e-15-heptadecenal, 1- newlineheneicosanol, hypophosphonousdichloride bis (1, 1-dimethylethyl) and heptadecyl newlinetrifluoroacetate. newlineThis study illustrates antidermatophytic lead compounds from S. albidoflavus and their newlinepurification and characterisation to explore the optimum dosage for antidermatophytic newlineactivity. It further progresses with the insilico molecular docking study of the seven GCMS newlinediscovered ligands and six dermatophytic modelled targets. newline