phytopharmacological investigation of neuracanthus sphaerostachyus for various inflammatory disorders

Abstract

Objective: newline newlineNeuracanthus sphaerostachyus Dalz has been traditionally used to treat skin diseases, diabetes, cough, asthma and indigestion. Lack of sufficient scientific evidence indicating the utility of this plant in the treatment of inflammation prompted us to investigate it for various inflammation associated conditions with different experimental screening methods. newline newlineMethodology: newline newlineVarious studies like microscopic analysis, macroscopic analysis and fluorescence analysis were carried out to identify the particular drug for surety of raw material. Preliminary phytochemical analysis and HPTLC study performed to support the previous findings and to set up phytochemical profile of plant. Acute oral toxicity study of aqueous and methanolic extract of Neuracanthus sphaerostachyus Dalz was carried out according to OECD 425 guideline in rat and mice and 3 doses were selected, 125, 250, and 500 mg/kg to reveal it s therapeutics effect. The present study investigated the protective effect of the methanolic and aqueous extract of Neuracanthus sphaerostachyus leaves against various inflammation associated disease conditions like ulcerative colitis and asthma. Different in vitro and in vivo screening methods like albumin denaturation technique, carrageenan induced paw edema, acetic acid induced increased vascular permeability, TNBS induced ulcerative colitis, compound 48/80 induced systemic anaphylaxis, compound 48/80 induced mast cell degranulation, milk induced leucocytosis and eosinophilia, inhibition of acetylcholine and histamine induced tracheal and ileum chain preparation were used to assess the therapeutic potential of plant. newline newlineResults and Discussion: newline newlineExtracts with 500 mg/kg doses were showed maximum % protection against different evaluated parameters. Higher doses were significantly effective in the management of various inflammation associated conditions. The anti-inflammatory activity of Neuracanthus sphaerostachyus Dalz was related to the presence of Flavonoids and other various potential phytoconstituents like Phenolic