pharmacological elucidation of juniperus communis in animal models of obesity and cholelithiasis

Abstract

Objective: newlineObesity is a metabolic disorder with limited FDA approved drugs to treat and standardized herbal plants have more potential and scope to treat such metabolic condition. Gallstone disease or cholelithiasis is a disease caused by change in bile secretions and cholesterol saturation to form crystal and blockage in bile duct. The current study focused on Pharmacological elucidation of Juniperus Communis leaves extract in various models of obesity and gallstone in mice model evaluate the anti-obesity effect of juniperus communis extract in various models of obesity. newlineMethodology: newlineVarious studies like microscopic analysis and macroscopic analysis were carried out to identify the particular drug for surety of raw material. Preliminary phytochemical analysis, HPTLC study and method validation (Luteolin content) performed to support the previous findings and to set up phytochemical profile of plant. the extracts were evaluated for various in-vitro test to access the potential of J. Communis leaves against different enzymes and on the basis of their phytochemical analysis and prescreening with enzymes the methanolic extract was selected to test in adipogenesis in 3T3L1 mouse adipocytes and other enzyme inhibition assay like Amylase, pancreatic Lipase, alpha glucosidase and hydrogen peroxide assay were also performed to determine the anti-diabetic activity. The cytotoxicity of extract was determined by the cell proliferation assay. Acute oral toxicity study of methanolic extract of Juniperus Communis was carried out according to OECD 425 guideline in rat and mice and 3 doses were selected, 150, 300, and 450 mg/kg to reveal it s therapeutics effect. High Fat Diet (HFD) induced obesity was developed in mice by administered HFD for a period of 8 weeks. The anti-obesity and anti-inflammatory effect of Methanolic extract of Juniperus cummunis (MEJC) extract was accessed by treating at three doses (150,300 and 450mg/kg, p.o.) mice for further 6 weeks. The various physiological and biochemical parameters like weight gain,