Optical Nano Fibre as a Biosensor and Anti cancer and Anti microbial activities of different metal oxide nano particles

Abstract

The objective of this study is to use an emerging device i. e Optical Nano Fiber, due to their biocompatibility, photo-sensitivity, as a biosensor which can detect any changes in cell and cellular proteins due the effect of mycotoxin produced by the Aspergillus sp. found in food and feed product of animals. First, we have collected the plasma (Blood without any cells) and treated the Plasma of human blood with different concentration of Aflatoxin B1 and Aflatoxin G1 (AflB1 and AflG1respectively) and after different time slot, we have studied the changes of the Plasma and its protein by Circular Dichorism spectroscopy (CD) and, Fluorescent Spectroscopy, Reactive Oxygen Species and many more. From these Spectroscopic data, it has been found that there was a minimal change of the blood protein, as AflG1 and AflB1 has found to get attached with the RBC (Red Blood Corpuscles) and change in the structure of the Hemoglobin. newlineMetal oxides are one of the major trace elements of the human body and co-factor of more than 300 mammalian enzymes, plays an important role in maintaining crucial cellular processes including oxidative stress, DNA replication, DNA repair, cell cycle progression and apoptosis. Thus, it is evident that an alteration in Copper levels in cancer cells can cause a deleterious effect. Research has shown that low Copper concentration in cells leads to the initiation and progression of cancer and high Copper concentration shows toxic effects. Copper-mediated protein activity disequilibrium and oxidative stress through reactive oxygen species (ROS) may be the probable mechanism of this cytotoxic effect. CuO has a neutral hydroxyl group attached to its surface, which plays an important role in its surface charge behaviour. Our aim is to show that the effect of Copper Oxide and Silica coated Copper Oxide on different microbes and cancer cells. Characterization of Cu nanoparticles have been done by using different analyzing techniques i.e. UV-Vis Spectroscopy, DLS (Dynamic Light Scattering) and SEM (Scanning Ele