Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/447428
Title: Isolation, characterization and pharmacological application of bioactive compound from leaves of Alpinia nigra (Gaertn.) B.L. Burtt
Researcher: Gupta, Manish Kumar
Guide(s): Rangan, Latha
Keywords: Biotechnology and Applied Microbiology
Life Sciences
Microbiology
University: Indian Institute of Technology Guwahati
Completed Date: 2022
Abstract: The present study is focused on investigating the potential uses of the herbaceous plant Alpinia nigra (Gaertn.) B.L. Burtt (Zingiberaceae). Traditionally this plant is used in folk remedies for curing gastritis and infectious diseases. The plant leaves are used as a food-flavoring agent by tribal people in Northeast (NE) India. However, the scientific community has not explored the plant leaf for its medicinal properties. Thus in the present study, the leaf extracts hexane (L-Hex), ethyl acetate (L-EtAc), and methanol (L-Met) were subjected to phytochemical analysis. The antibacterial, anti-biofilm, and anti-quorum sensing activities of the L-EtAc extract was determined by in-vitro analysis and was found to be potential. Further, the compound 3, 5-dihydroxy 4′,7-dimethoxy flavone (DHDM) was purified from L-EtAc, crystallized, and structural characterization was performed using multispectroscopic techniques including HRMS, FTIR, Raman, SC-XRD, 1-D NMR, and 2-D NMR. The cell viability assay showed no inhibition of DHDM at ≤200 μM concentration in THP-1 (human macrophage) and ≤80 μM in HaCaT (human keratinocyte) cell lines. Additionally, strong antioxidant properties and reduced ROS
Pagination: NA
URI: http://hdl.handle.net/10603/447428
Appears in Departments:DEPARTMENT OF BIOSCIENCES AND BIOENGINEERING

Files in This Item:
File Description SizeFormat 
01_fulltext.pdfAttached File9.47 MBAdobe PDFView/Open
04_abstract.pdf249.54 kBAdobe PDFView/Open
80_recommendation.pdf497.74 kBAdobe PDFView/Open
Show full item record


Items in Shodhganga are licensed under Creative Commons Licence Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0).

Altmetric Badge: