Protective Effect of Hydroethanolic Extract of Clematis Buchananiana Leaf on Diabetic Induced Neuropathy

Abstract

The present study was done to evaluate the Protective effect of hydroethanolic extract effect of Clematis Buchananiana leaf on diabetic induced neuropathy. Everybody knows that diabetes is a metabolic and lifestyle disorder. The occurrence of diabetes is due to increased blood sugar in the body when beta cells of pancreatic gland fail to produce enough quantity of insulin required by the body to neutralize the level of blood sugar in the body. Diabetic neuropathy which results due to increased blood sugar (observed commonly in diabetic patients) had emerged as one of the severe threats with clinical progression which is very difficult to treat. Its affects almost half of the population suffering from diabetes. The test drug prepared from the plant extract of Clematis Buchananiana leaves was investigated for evaluation of neuroprotective effect in diabetic-induced neuropathy in wistar rats. Two different experimental models were used for determination of inhibition pain. The first model used was Eddy s hot plate technique. In this method Wistar rats present in different experimental groups were placed one by one the surface of hot plate whose temperature of basal surface was maintained in the range of 50and#8451; to 55and#8451;. The basal reaction time was noted. The raw data obtained at the end of work was of significant value. The basal reaction time in control was the least, followed by the Test Group-1. Gabapentin, the standard drug gave maximum basal reaction time of 7.8 sec approx. (maximum in fourth week since day 1). After that the Test Group-3, showed significant response of 5.8 sec in final week whereas, the Test Group-2 also showed significant values after third week of treatment with a basal reaction time of 5 sec approximately. The other method used for evaluating the neuroprotective action was Tail flick analgesiometer. This method uses a radiant heat source for calculating the basal reaction time.