Studies on colorimetric estimation and fermentative purification of steviol glycosides

Abstract

The herb Stevia rebaudiana bertoni accumulates several sweet newlinetasting diterpene glycosides in its leaves The commercial importance of newlinestevia stems from the use of these glycosides as sugar substitutes newlineThe glycosidic linkages of the backbone steviol are at C13 and C19 The newlineglucose at the C19 is attached via a carboxyl group newlineThe first objective of this work was to develop a protocol for rapid newlinehigh throughput determination of steviol glycosides using simple newlineinstrumentation Current methods for analysis require expensive newlineinstrumentation and standards They are also not amenable for high newlinethroughput of samples newline newline