Transforming Growth Factor and#914;1 Promotes Runx2 Acetylation for Matrix Metalloproteinase 13 Expression in Osteoblastic Cells

Abstract

TGF-and#946;1 (transforming growth factor beta 1) functions as a coupling factor between bone development and resorption. Matrix metalloproteinase 13 (Mmp-13) is important in bone remodeling, and skeletal dysplasia is caused by a deficiency in Mmp-13 expression. Runx2, a transcription factor, is essential for bone development, and Mmp-13 is one of its target genes. TGF-and#946;1 promoted Runx2 phosphorylation, which was necessary for Mmp-13 production in osteoblastic cells, as we previously shown. Since the phosphorylation of some proteins causes them to be degraded by the ubiquitin/proteasome pathway, we hypothesized that TGF-and#946;1 promotes the activity of phosphorylated Runx2 by other post-translational modifications, such as acetylation. This study demonstrated that TGF-and#946;1 stimulated Runx2 acetylation in rat osteoblastic cells newline