Design synthesis and characterization of 8220 janus pnas 8221 novel backbone analogues that form double duplexes with cdna

Abstract

The thesis entitled 8216 8217 Design Synthesis and characterization of 8220 Janus PNAs 8221 Novel PNA backbone analogues that form double duplexes with cDNA 8217 8217 is comprised of studies towards the design synthesis biophysical evaluation of a new motif of peptide nucleic acid PNA analogs Janus PNA The modifications consist of incorporation of chiral azide triazole side chains at 945 position in the glycine segment of aminoethylglycine PNA backbone These side chains carry a second nuclobase at each monomer leading to backbone Janus PNAs The PNA oligomers synthesized on solid phase were investigated for their binding to target DNA by various biophysical techniques The work also describes the i Motif G quadruplex formation and stability of Janus PNAs and its hybridization studies with complementary DNA oligomers The thesis is presented as five chapters newline newline