Assessment of effect of curcumin On arecoline induced human buccal Mucosal fibroblasts a cell culture Study

Abstract

Introduction: Oral cavity is lined by the oral mucous membrane that is made up of an newlineoverlying epithelial layer and an underlying connective tissue. Fibroblasts are the newlineprinciple cells of the oral mucosa and buccal mucosa which in normal condition newlinesecrete collagen and elastin fibres. In pathological condition fibroblasts actively take newlinepart in wound healing. An abnormal increased secretion of collagen (Fibrosis) by the newlinecells is noted in both systemic and oral diseases. One of the common conditions newlineassociated with fibroblast pathologies is Oral sub mucous fibrosis (OSMF). OSMF is a newlinemulti-factorial pathological disorder in which arecanut plays a major role for the newlinepathogenesis of the precancerous condition affecting the fibroblasts at both cellular newlineand molecular level. Despite many treatment modalities like cessation of habit surgical newlineexcision, diathermy, the disease still remains irreversible .Recent clinical studies have newlineproved that the use of herbal products like curcumin, neem, tulsietc. have improved the newlinesigns and symptoms of OSMF. As curcumin is considered a safe natural product by newlineFood and Drug Administration (FDA), it becomes a choice of herbal product in newlineourstudy. newlineAims of the study: Our study aims to isolate the buccal mucosal fibroblasts from newlinehealthy individuals and assess the effect of arecoline, curcumin and curcumin on newlinearecoline induced cells for morphological and cytotoxic alterations newline