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http://hdl.handle.net/10603/429493
Title: | Structural and related studies on Mycobacterium tuberculosis pantothenate kinase |
Researcher: | Paul, Anju |
Guide(s): | Vijayan, M and Gopal, B |
Keywords: | Biophysics Life Sciences Molecular Biology and Genetics |
University: | Indian Institute of Science Bangalore |
Completed Date: | 2019 |
Abstract: | Pantothenate kinase (PanK) is a ubiquitous and essential enzyme that catalyses the first step in the universal Coenzyme A (CoA) biosynthetic pathway. In this step pantothenate is converted to phosphopantothentate, which subsequently forms CoA in four enzymatic steps. Three types of PanKs have been identified in bacteria, with variations in distribution, mechanism of regulation, cofactor requirement and affinity for substrates. As part of a major programme on mycobacterial proteins in our laboratory, studies on type I PanK from Mycobacterium tuberculosis (MtPanK) have been carried out previously. This investigation involved, apart from biochemical studies, structure determination of twenty-one independent crystals of binary and ternary complexes of MtPanK involving CoA, the ATP analogue AMPPCP, the GTP analogue GMPPCP, ADP, GDP, pantothenate, phosphopantothenate, citrate, pantothenol and nonyl pantothenamide. Analysis of these structures brought out the robustness of the mycobacterial PanK when compared to its E. coli homologue... newline |
Pagination: | 161 p. |
URI: | http://hdl.handle.net/10603/429493 |
Appears in Departments: | Molecular Biophysics Unit |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
01_title.pdf | Attached File | 119.97 kB | Adobe PDF | View/Open |
02_prelim pages.pdf | 280.93 kB | Adobe PDF | View/Open | |
03_table of contents.pdf | 156.59 kB | Adobe PDF | View/Open | |
04_abstract.pdf | 115.32 kB | Adobe PDF | View/Open | |
05_chapter 1.pdf | 1.73 MB | Adobe PDF | View/Open | |
06_chapter 2.pdf | 385.83 kB | Adobe PDF | View/Open | |
07_chapter 3.pdf | 1.08 MB | Adobe PDF | View/Open | |
08_chapter 4.pdf | 1.83 MB | Adobe PDF | View/Open | |
09_chapter 5.pdf | 1.88 MB | Adobe PDF | View/Open | |
10_annexure.pdf | 1.75 MB | Adobe PDF | View/Open | |
80_recommendation.pdf | 2 MB | Adobe PDF | View/Open |
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