Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/429214
Title: Regulation of Flagellar Motility in Giardia lamblia and Trichomonas vaginalis
Researcher: Sudhakar, Aparna
Guide(s): Tatu, Utpal
Keywords: Biochemistry and Molecular Biology
Biology and Biochemistry
Life Sciences
University: Indian Institute of Science Bangalore
Completed Date: 2021
Abstract: Protozoan pathogens are responsible for infections that are highly prevalent, especially in developing and under-developed countries. Flagellar motility is exhibited by a variety of organisms ranging from bacteria to certain cell types in mammals. In the context of pathogens, flagellar motility plays a significant role in the establishment of infection in the host. Unlike bacterial motility, where the mechanism and its contribution to pathogenesis is well studied, flagellar motility in protozoan parasites is an ill-explored field. Understanding the regulation of motility in these organisms would highly enhance our understanding of pathogenesis in these organisms and may possibly open new avenues of interventions. All eukaryotic flagella are made of microtubules and driven by dynein motor proteins. However, every organism is unique in terms of its flagellar waveform, beat frequency and its general motility pattern. In the current study, using biophysical and biochemical approach we studied the motility characteristics of Giardia lamblia and Trichomonas vaginalis, both flagellated protozoan pathogens which establish infection in the small intestine and the urogenital tract respectively. We have addressed the question of how flagellar motility may be differentially regulated in an organelle as structurally conserved as the flagella . We used a biophysical approach to first characterize the patterns of motility in both the organisms by combining microscopy and imaging with a high-speed camera to understand the movements of the cells to bring out the differences in terms of speed and beat frequency of the flagella. Our results demonstrate that despite overall conservation in flagellar structure, the pattern of tubulin post-translational modifications within the flagella are diverse and may contribute to variations in their patterns of motility. In this study, we have examined the tubulin post-translational modification in the protozoan parasites Giardia lamblia and Trichomonas vaginalis using global, untargeted m...
URI: http://hdl.handle.net/10603/429214
Appears in Departments:Biochemistry

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01_title.pdfAttached File38.17 kBAdobe PDFView/Open
02_prelim pages.pdf121.86 kBAdobe PDFView/Open
03_table of contents.pdf171.92 kBAdobe PDFView/Open
04_abstract.pdf137.2 kBAdobe PDFView/Open
05_chapter 1.pdf702.15 kBAdobe PDFView/Open
06_chapter 2.pdf241.88 kBAdobe PDFView/Open
07_chapter 3.pdf1.1 MBAdobe PDFView/Open
08_chapter 4.pdf1.16 MBAdobe PDFView/Open
09_chapter 5.pdf1.61 MBAdobe PDFView/Open
10_annexure.pdf611.4 kBAdobe PDFView/Open
80_recommendation.pdf158.32 kBAdobe PDFView/Open
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