Antimicrobial anti cancer activity and activation of intrinsic apoptotic pathway through taxifolin loaded zinc oxide nanoparticles in human breast cancer cells

Abstract

The current study is carried out with the aim of analyzing the anticancer newlineactivity of taxifolin loaded zinc oxide nanoparticles, against Human breast cancer cells, MCF-7 and to understand its mechanism of inhibitory action. The work is carried out in different distinctive phases. Materials like, Zinc oxide, Taxifolin, PEG coated and taxifolin loaded zinc oxide nanoparticles are synthesized by coprecipitation method and characterized by various methods. The drug releasing profile, loading and encapsulation efficiency of taxifolin loaded zinc oxide nanoparticles are assessed. Prolonged stability of synthesized taxifolin loaded nanoparticles is due to the presence of capping and stabilizing agent in the form of biomolecules, which is confirmed by FTIR analysis. Furthermore, the potentiality of synthesized zinc nanoparticles is done against pathogenic bacteria viz., Staphylococcus aureus and Escherichia coli, using the agar well diffusion method. On the basis of zone of inhibition, we can say that taxifolin loaded zinc oxide nanoparticles exhibited significant antibacterial properties. From the results it is found that, synthesized taxifolin loaded zinc oxide nanoparticles may be utilized for creation of antimicrobials and applied as potential microbial cell inhibitors. In order, to optimize the dose of synthesized zinc oxide nanoparticles in both the non-cancerous and cancerous cell lines, dose and time- dependent response studies are carried out. Further events of cell cycle are studied by using flowcytometry. Also, taxifolin loaded zinc oxide nanoparticles against antitumour activity in mice model are studied, and the expression of pro-apoptotic and anti-apoptotic proteins are evaluated by Western blotting analysis. newline newline