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http://hdl.handle.net/10603/424873
Title: | The Dual Role of Choline O sulfate on Chemical Denaturation of Proteins and Amyloid Aggregation A Computational Study |
Researcher: | Paul, Srijita |
Guide(s): | Paul, Sandip |
Keywords: | Chemistry Chemistry Applied Physical Sciences |
University: | Indian Institute of Technology Guwahati |
Completed Date: | 2020 |
Abstract: | This thesis represents the molecule choline-O-sulfate as a protecting osmolyte in the protein folding-unfolding process as well as a potent inhibitor in the field of peptide aggregation. The thesis is divided into seven chapters.Chapter 1 of the thesis includes a review of related experimental and theoretical works that exist in the literature together with the basic techniques of MD simulations. In Chapter 2, we have studied the synergistic behavior of urea-COS mixture through classical molecular dynamics simulation. Here we have studied all possible interactions between urea and COS present in a mixture to find out the mechanism of the counteraction of urea by COS against urea-induced denaturation of the protein. Chapter 3 deals with the direct application of COS as a protecting osmolyte in the protein folding-unfolding process. This chapter has been divided into two parts, Part A and Part B. Chapter 3A describes how COS nullifies the deleterious effects of urea on a 15 residue modeled peptide named S-peptide through classical molecular dynamics simulation. Chapter 3B includes the findings on the counteracting ability of COS against urea on a small globular protein called Trp-cage by an enhanced sampling method called Replica Exchange Molecular Dynamics (REMD) simulation. In the next chapter i.e. Chapter 4, we have discussed the unfolding of the terminal helices of the and#955;-repressor protein by an unconventional denaturant dodine and its stability in presence of COS. In Chapter 5, we have reported our research work on the inhibitory effects of COS in the aggregation of human islet amyloid polypeptide, responsible for Type-II diabetes mellitus (T2Dm). In Chapter 6, we have presented COS as a potent inhibitor in the self-association of Aand#946;16and#8722;22 peptide, associated with Alzheimer s disease. In the last chapter i.e., Chapter 7, we have summarized our overall findings to bring a concrete conclusion portraying COS as an efficient osmolyte as well as an inhibitor. Successful application of COS on two different kinds of pr |
Pagination: | Not Available |
URI: | http://hdl.handle.net/10603/424873 |
Appears in Departments: | DEPARTMENT OF CHEMISTRY |
Files in This Item:
File | Description | Size | Format | |
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01_fulltext.pdf | Attached File | 6.71 MB | Adobe PDF | View/Open |
04_abstract.pdf | 68.48 kB | Adobe PDF | View/Open | |
80_recommendation.pdf | 886.03 kB | Adobe PDF | View/Open |
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