Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/423839
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dc.date.accessioned2022-12-09T10:54:01Z-
dc.date.available2022-12-09T10:54:01Z-
dc.identifier.urihttp://hdl.handle.net/10603/423839-
dc.description.abstractThe present work provides an insight into the concept, development, and application of the whole-cell biosensors for organophosphorus pesticide determination. For the fabrication of biosensor, Chlorella sp. algal cells having phosphatase activity were chosen. The reason for choosing algal cells as a sensor-modifying agent was their stability in quasi-physiological conditions, easy to handle, economical, high productivity and have a longer life span compared to bacterial or other whole cells. It is necessity for a biosensor that biological recognition element and transducer should work as a single unit, thus, immobilization becomes an important step for biosensor development. Our work has primarily focused on the fabrication of electrochemical biosensors. The algal cells with anticipated phosphatase enzyme were immobilized on the electrode surface using appropriate techniques. The immobilization technique solely depends upon the type of electrode used for preparation of biosensor. The thesis has been divided into two sections- (i) Inhibition based biosensors, (ii) Catalytic biosensors. Inhibition based biosensor are those in which pesticide concentration is determined by measuring the extent of inhibition of the enzyme activity. To increase sensitivity, operational stability, and to attain low detection limit, ZnO nanostructures have been introduced in the design of biosensor. Two types of biosensors have been developed under this section: (i) An electrochemical biosensor for the determination of acephate pesticide was developed based on algal modified silica coated ZnO quantum dots (QDs). The surface active alkaline phosphatase enzyme on the Chlorella sp. algae cell wall dephosphorylates substrate p-nitrophenyl phosphate (pNPP) and release p-nitrophenol (pNP), which being electroactive gets oxidized on the modified working electrode to generate current equivalent to the amount of pNP released from the substrate.
dc.format.extentix, 110p.
dc.languageEnglish
dc.relation
dc.rightsuniversity
dc.titleDevelopment of Some Biosensors and Their Analytical Applications
dc.title.alternative
dc.creator.researcherPabbi, Manisha
dc.subject.keywordPesticide
dc.subject.keywordPhysical Sciences
dc.subject.keywordWhole cell biosensors
dc.subject.keywordZnO nano particles
dc.description.note
dc.contributor.guideMittal, Susheel
dc.publisher.placePatiala
dc.publisher.universityThapar Institute of Engineering and Technology
dc.publisher.institutionSchool of Chemistry and Biochemistry
dc.date.registered
dc.date.completed2019
dc.date.awarded2020
dc.format.dimensions
dc.format.accompanyingmaterialNone
dc.source.universityUniversity
dc.type.degreePh.D.
Appears in Departments:School of Chemistry and Biochemistry

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01_title.pdfAttached File100.88 kBAdobe PDFView/Open
02_prelim pages.pdf1.38 MBAdobe PDFView/Open
03_content.pdf266.48 kBAdobe PDFView/Open
04_abstract.pdf167.27 kBAdobe PDFView/Open
05_chapter 1.pdf444.3 kBAdobe PDFView/Open
06_chapter 2.pdf384.94 kBAdobe PDFView/Open
07_chapter 3.pdf458.43 kBAdobe PDFView/Open
08_chapter 4.pdf4.72 MBAdobe PDFView/Open
09_annexures.pdf148.33 kBAdobe PDFView/Open
80_recommendation.pdf4.82 MBAdobe PDFView/Open


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