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http://hdl.handle.net/10603/422558
Title: | Identification of systemic risk factors and genetic biomarkers for intravitreal steroid induced ocular hypertension |
Researcher: | Lakshmi, B |
Guide(s): | Lakshmi, B S |
Keywords: | Genetic Biomarkers Intravitreal steroid Hypertension |
University: | Anna University |
Completed Date: | 2022 |
Abstract: | Initially, to determine the incidence and risk factors associated with newlineSI-OHT in the Indian population, a retrospective study was performed to newlineinvestigate the steroid response upon 1549 TA and Dex administrations in newline1075 eyes of 897 patients at a tertiary ophthalmic hospital, Chennai, India. newlineIOP values were extracted from the hospital s electronic medical records newline(EMR) before steroid administration (baseline IOP), day-1, month-1, month- newline3, and month-6 post injections. Glaucoma patients and suspects, uveitis, newlinetrauma cases were excluded from the study. Patients were categorized as newlinesteroid-responders (SR): IOP and#8804; 21mmHg and steroid non-responders (NR): newlineIOP and#8804; 20mmHg based on the IOP values. The incidence rate, the time and newlinemagnitude of the IOP rise, and treatment details were compared between TASR newlineand Dex-SR cohorts. Ocular and systemic parameters associations with newlineSI-OHT were compared between SR and NR of TA-treated and Dex-treated newlinegroups. Further, to identify the genetic and molecular determinants associated newlinewith SI-OHT, blood samples were collected from 66- TA and 60-Dex treated newlinesubjects after obtaining informed consent. Plasma and DNA samples were newlineisolated. An enzyme-linked immunosorbent assay (ELISA) was performed to newlineidentify the role of eight circulatory inflammatory cytokines in SI-OHT newlineprogression from the isolated plasma samples. Further, the genetic newlinedeterminants associated with SI-OHT were identified based on genotyping of newlineknown SNPs identified among Caucasian subjects and the GWAS for novel newlineSNP identification identification among Indian subjects. Thereby a newlinepreliminary validation study for the 48 SNPs identified from the Caucasian newlinepopulation was performed among TA (n=54) and Dex (n=47) treated Indian newlinepatients. In this analysis, the genotypic frequencies were calculated and a newlinesignificant association with OHT was compared between the following newlinegroups; a) steroid-treated versus reference healthy controls; b) SR versus NR; newlinec) influence of time, severity, and gender on IOP rise. newline |
Pagination: | xxi,194p. |
URI: | http://hdl.handle.net/10603/422558 |
Appears in Departments: | Faculty of Science and Humanities |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
01_title.pdf | Attached File | 235.7 kB | Adobe PDF | View/Open |
02_prelim pages.pdf | 605.41 kB | Adobe PDF | View/Open | |
03_content.pdf | 409.96 kB | Adobe PDF | View/Open | |
04_abstract.pdf | 272.18 kB | Adobe PDF | View/Open | |
05_chapter 1.pdf | 1.06 MB | Adobe PDF | View/Open | |
06_chapter 2.pdf | 679.82 kB | Adobe PDF | View/Open | |
07_chapter 3.pdf | 3.39 MB | Adobe PDF | View/Open | |
08_annexures.pdf | 2.29 MB | Adobe PDF | View/Open | |
80_recommendation.pdf | 66.49 kB | Adobe PDF | View/Open |
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