Investigating the structure function and dynamics of an intrinsically disordered enzyme

Abstract

The engineered intrinsically disordered enzyme monomeric chorismate mutase mCM catalyses the rearrangement of chorismate to prephenate and hence emerged as a valuable model system to study enzymatic catalysis mCM displayed all the hallmarks of a molten globule a functionally inactive protein folding intermediate but surprisingly shows near native catalytic activity The nature of structural plasticity mechanisms in mCM that enable catalysis were investigated in this thesis work We probed...