Pre Clinical Investigation on Antipyretic Activity of Guduchi Tinospora Crispa L Hook F and Thomson Sattva and its Pharmaceutical Modification as Sublingual Nano Film

Abstract

Guduchi sattva is a well-known Ayurvedic formulation explained in Yogaratnakar/ Rajayakshma Chikitsa. The method of preparation is clearly explained in Ayurveda Sara Sangraha. The Guduchi species considered for the present study is Tinospora crispa L.Hook f Thomson. As per the reference it is mainly used for treating Teevra tapa (Pyrexia), jwara(Fever), raktapitta (bleeding disorders), rakta pradara (Uterine haemorrhage) etc. The dose explained in classics is 4ratti to 1 masha (500mg-1g). Initially Guduchi sattva will be prepared as per the Ayurveda Sara Sangraha reference by collecting Guduchi (Tinospora crispa L. Hook f Thomson.) of Meninspermiaceae family. Then the Guduchi sattva analytical evaluation will be performed as per API. Thus the present study is pointed into two research angles, one is to evaluate the antipyretic activity of Guduchi sattva in albino rats against yeast induced pyrexia and the second part is to modify the Guduchi sattva into a Sublingual Nano Film. In pharmacological area four group of six animals each will be used for the experiment. The yeast induced pyrexia method will be standardised first by injecting 12.5% yeast suspension (s.c) followed by recording the rectal temperature at regular intervals as per the article references. The second part of the study will be the pharmaceutical modification as newlineSublingual Nano Film. The need for modification in the present study is to minimise dose, rapid action and to increase the bioavailability. The maximum dose which can be incorporated in a film will be 20mg.Initially a nanosuspension will be prepared by using high speed homogenizer, Ultra sonicator etc. Based on the 32 Factorial design the optimization of the film will be done. By doing continuous pilot studies the ratio of the ingredients was fixed. HPMC E 15 and PEG were used to stabilize the nanosuspension. HPMC E 15 is a key ingredient in formulation of film which rapidly disintegrate in presence of water or saliva.