Shodhganga : a reservoir of Indian theses @ INFLIBNET
The Shodhganga@INFLIBNET Centre provides a platform for research students to deposit their Ph.D. theses and make it available to the entire scholarly community in open access.
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10603/419790
| Title: | Cloning expression process analytical technology pat enabled monitoring and control of glycoengineered pichia pastoris cultivation for human interferon 945 2b production |
| Researcher: | Srikanth, Katla |
| Guide(s): | Sivaprakasam, Senthilkumar |
| Keywords: | Biochemistry and Molecular Biology Biology and Biochemistry Life Sciences |
| University: | Indian Institute of Technology Guwahati |
| Completed Date: | 2019 |
| Abstract: | Recombinant huIFN 206 177 2b has been expressed on various platforms which includes bacteria yeast insect cell lines mammalian cell lines and plants Expression of recombinant huIFN 206 177 2b in E coli yielded high huIFN 206 177 2b titer but the expressed protein forms inclusion bodies and loses the activity during renaturation process Also the expressed protein lacks post translational modifications and exhibited very less plasma life The plasma life of huIFN 206 177 2b expressed by E coli increased to certain e... |
| Pagination: | Not Available |
| URI: | http://hdl.handle.net/10603/419790 |
| Appears in Departments: | DEPARTMENT OF BIOSCIENCES AND BIOENGINEERING |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 01_fulltext.pdf | Attached File | 9.01 MB | Adobe PDF | View/Open |
| 04_abstract.pdf | 211.2 kB | Adobe PDF | View/Open | |
| 80_recommendation.pdf | 529.88 kB | Adobe PDF | View/Open |
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