Engineering Peptide Conjugated Nanoparticles by using Versatile Di block Copolymers for Efficient Tumour Targeting Delivery System

Abstract

In clinic, when patients have been diagnosed with cancer, metastasis had already existed in most cases. Even for those patients without detectable metastasis, micro metastasis will eventually lead to a severe disease status after the removal of primary cancer by surgery. Therefore, to control the metastasis beyond primary tumor is a very important concern to prevent the progression of cancer. Systemic chemotherapy, which is considered to be one of the most effective ways to control metastasis at present, has been used with little success because most of the currently used drugs delivered in this way are quickly destroyed or inactivated in blood and liver after administration. Therefore, in the metastatic setting, one must carefully weigh toxicity vs. the possibility of survival prolongation. During the various stages of metastasis, metastasizing tumor cells encounter various host cells and/or extracellular matrix (ECM) and basement membrane components. Tumor metastasis is the one of the major causes of morbidity and mortality in patients with solid malignant tumor and is the result of successive interactions between cancer cells and host tissues. Therefore, the compound which can interact with cell surface receptors and competitively inhibit the interaction between tumor cell and extracellular matrix are clinically useful. Peptide YIGSR-NH2 and EILDV-NH2 derived from protein laminin and fibronectin respectively responsible for binding to laminin and integrin receptors was utilized as ligand on the surface of polymeric micelles (PEG-PCL) loaded with anticancer drug Etoposide. YIGSR-NH2/ EILDV-NH2 conjugated micelles were showed their potential in effective treatment of metastasis when evaluated in-vitro using highly metastatic B16F10 cell line and proved that the formulated drug delivery carrier helps in reduction in metastasis of tumor compared to plain drug. Overall, this carrier system brings us a step closer with use of antimetastatic peptide like YIGSR-NH2 and EILDV-NH2 in prevention and treatment of metastasis.