Production Optimization and Characterization of Active Metabolites from Marine Yeasts

Abstract

ABSTRACT newlineOleaginous yeasts are a promising solution for lipid production which is rich in nutritional value and can be used in the production of bio-fuels, value added products and pharmaceutical protein production. This study outlines an efficient and much competitive approach of analyzing the marine deep sea water for lipid production and identified accumulation of lipids. In this study oleaginous yeasts from deep-sea water were isolated and extracted lipids through gravimetric method. The marine water was collected from Bay of Bengal at different depths of 2m, 50m, 500m, 1500m, 2000m and 2500m from three different stations. Out of 65 yeasts, three were identified to accumulate more than 30% of lipids and selected for further studies. C:N ratio, and the environmental parameters such as pH and temperature were optimized. The isolates showed maximum lipid accumulation after 72 hrs of 10% inoculation. The lipids were characterized using FTIR and GCMS analysis. The identified strains belong to the species of Meyerozyma and Candidawhich can be used for commercial application in the field of aquaculture. The TI, LC50 and EC50values in zebra fish toxicity studies of oleaginous yeasts confirmed the potential application of the yeast strain OY53 in aquatic supplementary studies. newline