Studies on The Effect of Beta Sitosterol on Insulin Signaling Molecules and Glucose Oxidation in Liver of High Fat Diet and Sucrose Induced Type 2 Diabetic Adult Male Rats

Abstract

ABSTRACT newlineChanges in lifestyle, such as high-calorie diet intake and lack of exercise, have increased the global prevalence of obesity and diabetes. The major pathophysiological event contributing to the development of type 2 diabetes mellitus is the target tissues to insulin action. Liver is the largest organ of the body that carries out all important metabolic processes of carbohydrate, protein, and lipid. It plays an important role in the regulation of glucose hemostasis. and#946;-sitosterol, a naturally occurring plant sterol, ubiquitously found in many plants. The study was aimed at assessing the effect of and#946;-sitosterol on insulin signaling molecules and glucose oxidation in the liver of high fat diet and sucrose induced type 2 diabetic adult male rats. Experimental Design: Adult male Wistar rats were used and divided as Group I: Control; Group II: Type-2 diabetic rats; Group III: Type-2 diabetic rats treated with and#946;-sitosterol and Group IV: Type-2 diabetic rats treated with metformin. After 30 days of treatment, animals were anesthetized; liver tissues were dissected out and used for the assessment of fasting blood glucose, oral glucose tolerance test, fasting serum insulin, insulin tolerance test, HOMA-IR, QUICKI, carbohydrate metabolic enzymes, antioxidant enzymes ,lipid profile, renal profile, liver enzymes, lipid peroxidation, glucose uptake , glucose oxidation, IRS protein and its mRNA expression, IRS 2 protein and its mRNA expression and GLUT2.CONCLUSION:The study highlighted, that the antidiabetic effect of and#946;-sitosterol in high fat diet and sucrose-induced type-2 diabetic rats were mediated through amelioration of oxidative stress and dyslipidemia, improved insulin signal transduction and GLUT2 expression as well as glucose uptake and oxidation in the liver. newline newline