Study on fabrication of silk fibroin Paramylon based biomimetic and Instructive materials for Chronic wound healing

Abstract

Chronic wounds are one among the dangerous issues that are predicted to affect 2-6 crore people around the world by 2026 due to the endless rising of obesity, aging, diabetes and change in lifestyle. These wounds are more than just a cost burden, as they create immobility and worthlessness of life due to the disability to perform daily tasks. Factors responsible for the chronicity in wounds are continuous trauma, debilitating illness, increased bacterial protein, ischemia, excessive proteases, destruction of extracellular matrix (ECM) and growth factors that are mandatory for healing. Thus, to positively regulate the proteases like Matrix metalloproteases and to activate the growth factors and ECM, Biomimetics is one of the favourable approaches to develop an ECM like dressing to progress the wound healing process in chronic wounds. Due to the increasing interest and the ECM framework, Protein-Polysaccharide complex driven the curiosity in developing a biomimetic and instructive wound dressing usind silk fibroin protein and Paramylon polysaccharide-based complex of different forms have been studied to develop a self-care wound dressing for chronic wounds. Primarily to study the suitability, silk fibroin and paramylon blended films was prepared in different ratio (100:0, 75:25, 50:50, 75:25 and 0:100). Later three forms of Silk fibroin and paramylon-based materials was fabricated such as (1) Silk Fibroin based non-woven mat functionalized with 0.25%, 0.5% and 1% paramylon (2) Alum loaded FG 75:25 coated cellulose sponge for exudate wounds and (3) Paramylon coated silk fibroin sponge sheet. The surface topography, molecular confirmation, wettability, porosity, swelling capacity, enzymatic degradation, hemolysis and cytotoxicity studies were studied for all three forms. In which, one from each was considered to iv study the wound healing activity along with the paramylon release kinetics. newline